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Mammalian cell colony counting > GelCount > Screenshots  
   

The GelCount colony counting platform includes highly sophisticated image analysis software presented within an intuitive, efficient and flexible interface.

The following screenshots highlight some of the primary GelCount software features:

 

 

 


Multi-well plates are viewed one well at a time with simple zoom and ‘panning’ controls providing intuitive image navigation so that any region can be quickly viewed at high resolution for close-up verification of colony detection. Detected colonies are highlighted by red, size-proportional markers. The current colony count and related colony statistics such as mean colony diameter are displayed in a summary panel in the left margin.

 



 


Not only does GelCount automatically detect and count colonies but it is also capable of calculating and presenting in histogram format a unique set of statistical distributions, such as for colony size and OD per well or dish. Histogram data can be manually or automatically exported in bitmap or in Excel®-compatible raw data formats.

 





Colony detection is fully user-definable from within the novel 'CHARM Optimizer' utility, which allows adjustment of numerous sensitivity and shape-related parameters and, crucially the ability to include or exclude colonies based on size.

The effect of any such adjustments can be quickly previewed and parameters stored for re-use in future experiments. GelCount achieves outstanding counting proportionality and reproducibility by objective and consistent application of these user-defined settings.

 





GelCount's data storage options are plentiful, from basic colony counts and mean per-well/dish colony statistics to full digital image archiving and per-colony raw data exportation. Summary data, including colony counts and colony statistic means are exported directly into Microsoft Excel®.

 




Images of colony plates or dishes can be saved in a general-purpose bitmap format (for printing, presentations etc) or in a raw image format, which supports processing or re-processing of samples in ‘off-line’ mode on any independent workstation with the software installed.

 



 

 

 

 

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