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| Mammalian
cell colony counting > GelCount > Screenshots |
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| The GelCount colony
counting platform includes highly sophisticated image analysis
software presented within an intuitive, efficient and flexible
interface.
The screenshots below provide
a sneak preview at the user-interface and introduce some of
its primary features:
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Multi-well
plates are viewed one well at a time with simple zoom
and ‘panning’ controls providing intuitive
image navigation so that any region can be quickly viewed
at high resolution for close-up verification of colony
detection. Detected colonies are highlighted by red,
size-proportional markers. The current colony count
and related colony statistics such as mean colony diameter
are displayed in a summary panel in the left margin.
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Not
only does GelCount automatically detect and count colonies
but it is also capable of calculating and presenting
in histogram format a unique set of statistical distributions,
such as for colony size and OD per well or dish. Histogram
data can be manually or automatically exported in bitmap
or in Excel®-compatible raw data formats.
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Colony detection is
fully user-definable from within the novel 'CHARM Optimizer'
utility, which allows adjustment of numerous sensitivity
and shape-related parameters and, crucially the ability
to include or exclude colonies based on size.
The effect of any such adjustments
can be quickly previewed and parameters stored for re-use
in future experiments. GelCount achieves outstanding
counting proportionality and reproducibility by objective
and consistent application of these user-defined settings.
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GelCount's data storage options are plentiful, from
basic colony counts and mean per-well/dish colony statistics
to full digital image archiving and per-colony raw data
exportation. Summary data, including colony counts and
colony statistic means are exported directly into Microsoft
Excel®.
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Images of colony plates or dishes can be saved in a
general-purpose bitmap format (for printing, presentations
etc) or in a raw image format, which supports processing
or re-processing of samples in ‘off-line’
mode on any independent workstation with the software
installed.
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