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Mammalian cell colony counting > ColCount > Screenshots  
   

Comprehensive Windows-compatible software (in version 5 as of March 2009!) provides an intuitive yet highly sophisticated image processing and colony analysis solution.

The screenshots below provide a sneak preview at the user-interface and serve to introduce some of the primary software features:

 


For every dish or flask analyzed, ColCount displays the image and highlights all detected colonies on the image by red, size-proportional triangular markers. The colony count and related colony statistics such as mean colony diameter are displayed in a summary panel in the left margin. Standard zoom controls and ‘panning’ functionality are available to view selected regions of the dish or plate close up.



 


Individual colony ‘statistics’, such as colony diameter can be called up and viewed in the form of a histogram for a given dish or flask. Histogram data can be manually or automatically exported in bitmap or in Excel®-compatible raw data formats.






A representative sub-region of the dish or flask can be selected for the generation of customized colony detection parameters. The selection is viewed within the ‘CHARM Optimizer’ which allows extensive control over colony detection via adjustment of numerous parameters including the ability to include or exclude colonies based on definable diameter thresholds.

Custom detection settings are stored to disk as templates and can be re-called. ColCount achieves outstanding counting proportionality and reproducibility by objective and consistent application of these user-defined settings.

 






The Data exportation options include direct exportation of summary data such as colony count and mean diameter information to Excel®.

ColCount also provides options for saving images of dishes/flasks in bitmap or in raw image formats. The raw image format supports processing or re-processing of samples in ‘off-line’ mode on any independent workstation with the software installed.



     

 

 

 

 

 

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