Software update and an siRNA library screening protocol
After months of effort by our developer team, we are excited to announce our latest software release for GelCount.
Version 18.104.22.168 brings a ton of new functionality as well as ‘behind the scenes’ changes that are preparing the ground for the exciting plans we have in store for this platform.
• A new "Centres only" function that decouples shape fitting from colony/object detection, provides improved object detection performance in high density / small object scenarios (e.g., single cells, or colonies/objects below 50 um diameter)
• Implementation of the FreeImage imaging library, and other ‘behind the scenes’ changes to support future plans
• Improved support of parallel processing, for enhanced utilization of modern high core-count CPUs
• Improved memory efficiency and implementation of a memory limit guard
• Expanded choice of 'generic' image export formats (.bmp, .jpg, .tif, or .png)
• New “Data Plate Layout” numerical data output option
• The ICS raw image file format is now associated with GelCount; double-click an ICS image to view in GelCount software
• Improved support for non-circular masks, now selectable via a toolbar pull-down
• Improved auto-mask detection
• Mask shape controls extended for T25 flasks
• Miscellaneous bug fixes
One of our customers based in Oxford, UK recently published an interesting protocol utilizing the GelCount, which describes an automated 96-well format high-throughput colony formation assay for screening siRNA molecular libraries in HeLa cervical cancer cells.
For those interested, the article can be found here.
Florey Institute of Neuroscience and Mental Health, Melbourne, Australia
HypoxyLab, but better!